Job Title: Senior Associate Scientist, Biochemistry

Work Location: Middlesex County, Massachusetts

Summary:

Seeking a motivated Senior Associate Scientist to support scalable biochemical assay testing and recombinant protein production. This contract role involves routine compound testing, protein expression and purification, and collaboration with cross-functional teams to deliver high-quality data for discovery programs.

Responsibilities:

• Conduct routine biochemical assays for discovery compounds, including plate-based and label-free techniques, ensuring accurate compound handling and quality control.
• Maintain assay consistency by applying controls, reference standards, and monitoring key performance metrics.
• Support rapid structure-activity relationship studies by aligning compound testing workflows with project requirements.
• Document all assay procedures and results in electronic notebooks and manage data uploads to internal platforms.
• Prepare and present assay data for team meetings, clearly communicating results and any observed anomalies.
• Independently design, express, and purify recombinant proteins from various cell systems, performing thorough quality control before release.
• Troubleshoot technical issues in assays and protein production, proposing and implementing process improvements within established protocols.
• Assist with reagent qualification, method transfers, and maintain laboratory spaces, equipment, and inventory to support ongoing research activities.

Qualifications:

• Bachelor’s degree in Biology, Biochemistry, Molecular Biology, Bioengineering, or related field with approximately 6+ years of relevant industry experience, or Master’s degree with 2-4+ years of relevant experience.
• Hands-on experience with E. coli, mammalian, and insect cell culture systems.
• Proficiency in protein purification methods, including affinity chromatography and preparative SEC.
• Experience with protein biochemical and biophysical techniques such as FP, TR-FRET, SPR, analytical SEC, SDS-PAGE, and western blotting.
• Skilled in executing plate-based assays, including use of multichannel pipettes and/or liquid handling systems.
• Strong attention to detail, data quality, and consistent documentation; comfortable working with SOPs and timelines.
• Effective communication skills and ability to collaborate in a cross-functional, on-site environment.
• Preferred: Experience with liquid chromatography-mass spectrometry assays, oncology or peptide modalities, automation (liquid handlers), high-throughput assay operations, and assay QC statistics.

Published Category: Discovery Research & Preclinical